TRANSMISSION ELECTRON MICROSCOPY

BIOLOGY 221



CATALOG DESCRIPTION

BIO 221: Transmission Electron Microscopy - 4 credits
Prerequisites: BIO 109-110 or equivalent, CHE 151-152 or equivalent.
An introduction to the basic principles of transmission electron microscopy including tissue preparation, microscope (TEM) operation, black & white photography, and micrograph interpretation. The entire laboratory is devoted to the development of skills and preparative techniques involved with the operation of an actual transmission electron microscope.
(3 lecture, 3 laboratory hours)
Laboratory fee applies.

COURSE SYLLABUS

WEEK 1:
Introduction, Basic EM Concepts and History, Lab Tour, Technique
Orientation (Block Trimming & Glass Knife Making)

WEEK 2:
Ultramicrotomy Lecture; Demonstrations of Block Trimming, Knife Making
& Evaluation, Knife Boating, Thick and Ultra-thin Sectioning, Grid Cleaning

WEEK 3:
B&W Photography Lecture, Solution Preparation (D-19, Dektol, Fixer),
Negative Handling and Development, Printing (Exposure, Tricks,
Development, Washing, Drying)

WEEK 4:
TEM Principles Lecture, Start-Up Procedure, Specimen Loading, Focusing,
Plate Photography (Exposure Time, Loading Plate Box, Removing and
Replacing Receiver Box), Shut-Down Procedure, High Vacuum Systems

WEEK 5:
TEM Alignment Lectures, Alignment Demonstration
Astigmatism Correction (Holey Grid), Fixation Lecture

WEEK 6:
Tissue Fix & Embed Protocol, Resins Lecture

WEEK 7:
Technique Practice, TEM Orientations (One-on-One)

WEEK 8:
Theory Exam & Open Lab

WEEK 9:
Practicals, Grid Coating Demo (Formvar), Open Lab

WEEK 10-14:
Open Labs, Practicals, Filament Change Demo, Analysis Lecture

WEEK 15:
Final TEM Micrograph Presentations, Critiques, Clean-Up


Assignment: Library Research - TEM Protocols Due Prior To WEEK # 5

Requirements:
1. Theory Exam
2. Practical Ability (Ultramicrotomy and TEM)
3. Micrographs (Minimum 0f 4 - 2 Low Mag & 2 High Mag)
Micrograph Quality is of major importance in determining the final grade!
4. Patience (a virtue in life, a necessity in EM)


COURSE OFFERING

The TEM course (BIO 221) is offered every Fall semester on Thursday evenings starting at 5:30 p.m. The course is also offered during Summer Session I from 8:00 a.m. until noon, Monday through Thursday. The course can also be offered during the Spring day session by student demand, usually MW from 8:30 a.m. until 11:15 a.m.

Consult the NCC homepage for current course schedules.


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