SCANNING ELECTRON MICROSCOPY

BIOLOGY 222



CATALOG DESCRIPTION

BIO 222: Scanning Electron Microscopy - 4 credits
Prerequisites: BIO 221 or permission of the instructor
An introduction to the theoretical and practical concepts of biological scanning electron microscopy (SEM). Topics studied include SEM optical principles, preparative techniques, SEM photographic techniques, SEM micrograph analysis. Through "hands-on" SEM operation, students will produce a portfolio of micrographs of a variety of biological samples.
(3 lecture, 3 laboratory hours)
Laboratory fee applies.

COURSE SYLLABUS

WEEK 1:
Introduction, Basic EM Concepts and History, Lab Tour & SEM Demo.

WEEK 2:
Electron Optical Principles, Limits of Resolving Power, Imaging Principles,
SEM Design, Specimen-Beam Interactions, High Vacuum Systems.

WEEK 3:
SEM Operation, Start-Up Procedure, Specimen Loading (Stage), Filament
Energizing, Main Controls & Parameters, Negative/Polaroid