SCANNING ELECTRON MICROSCOPY
BIO 222: Scanning Electron Microscopy - 4 credits
Prerequisites: BIO 221 or permission of the instructor
An introduction to the theoretical and practical concepts of
biological scanning electron microscopy (SEM). Topics studied
include SEM optical principles, preparative techniques, SEM
photographic techniques, SEM micrograph analysis. Through
"hands-on" SEM operation, students will produce a
portfolio of micrographs of a variety of biological samples.
(3 lecture, 3 laboratory hours)
Laboratory fee applies.
- WEEK 1:
- Introduction, Basic EM Concepts and History, Lab Tour
& SEM Demo.
- WEEK 2:
- Electron Optical Principles, Limits of Resolving Power,
SEM Design, Specimen-Beam Interactions, High Vacuum
- WEEK 3:
- SEM Operation, Start-Up Procedure, Specimen Loading
Energizing, Main Controls & Parameters,