BIO-IMAGING CENTER
ELECTRON MICROSCOPY LABORATORY

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    The Electron Microscopy Laboratory has existed in the Department of Biology at NCC since 1970. The first instrument obtained in that year, which is still operational, was a Hitachi HS-8-2 Transmission Electron Microscope (TEM). The instrument was obtained through a vocational education (VEA) grant which led to the development of a course in Transmission Electron Microscopy (BIO 221) by Dr. James Averett. Dr. Averett initially offered faculty instruction in the use of the TEM followed by offering the course to students for the first time in the spring 1979 semester. Prof. Stephen Beck, the current course coordinator, has been teaching the course since the spring 1986 semester. In 1995, a Philips EM-300 (TEM), was donated to NCC by the EMSL corporation. Installation is expected in the spring 1996 semester for use in the future BIO 221 courses.

    Recently (April, 1992), the department was fortunate to acquire a new, state-of-the-art, Scanning Electron Microscope (SEM), a Hitachi S-2400, through a matching
    National Science Foundation, Instrumentation for Laboratory Improvement (ILI) grant, coauthored by Prof. Beck and Dr. Baruch May. As a result of the new SEM, Prof. Beck has developed a course in Scanning Electron Microscopy (BIO 222) which has been offered and run very successfully numerous times, beginning with the 1992 summer session. Another aspect of the grant project has been the integration of electron microscopy into the biology department curriculum. Currently, the integration process is well underway, with Prof. Beck providing guided tours of the facility to many departmental classes at all levels. Prof. Beck also provides tours to area middle schools and high schools. The final aspect of the grant project involves the development of an A.S. program in Electron Microscopy/Biological Research Technology which is being coordinated by Prof. Beck and Dr. Patricia Cassin.



    Since courses in electron microscopy are generally available only at the graduate level, our students are provided with a unique opportunity to view cellular structure at the molecular level, and from an understanding of molecular structure, to better comprehend biological function/processes. Both TEM and SEM courses are taught with a theoretical and "hands-on", skills emphasis. This approach provides students who complete the courses, the competence to function as technicians in an electron microscopy biological research laboratory.

    Both instruments (TEM/SEM) utilize a high voltage generated electron beam and electromagnetic lenses, to focus the beam, in order to yield high resolution, detailed images at the near atomic/molecular level.

    Since the mid 1950's, with the refinement of biological specimen preparatory techniques, the TEM has revolutionized the way biologists view and understand the cell. The word "Transmission" indicates that the electron beam passes through the specimen such as light passes through the specimen in a light microscope. In order that the electron beam pass through the specimen, the samples must be cut ultrathin, less than 1/10,000 of a millimeter, using a diamond or glass fracture knife and a precision machine called an ultramicrotome. The specimens are stained using heavy metals in order to generate contrast on the TEM fluorescent viewing screen. The techniques of specimen preparation are tedious with the utmost of care taken to avoid contamination. In the BIO 221 course, students acquire these skills and put them to use on their required projects.

    In the mid 1960's, the SEM became available commercially. Using a beam of electrons which scans the surface of a specimen, the SEM produces an indirect image of the surface based on specimen topography/relief, on a cathode ray tube (TV monitor). Interaction of the primary electron beam with the specimen surface causes the generation of what are known as secondary electrons (and other energetic phenomena), or the signal. The secondary electron signal is collected and channeled to the viewing CRT in order to form a point by point image. The generation of contrast (dark vs. light vs. gray areas) is based on specimen surface topography. High areas of relief are contacted most directly by the primary electron beam and produce greater signal, therefore appearing brighter than low areas of relief, which appear darker on the viewing screen. Due to an extremely high depth of field in the SEM, the high resolution images appear virtually three-dimensional.

    Specimen preparation for SEM is generally less involved than for TEM. Since the electron beam does not have to pass through the specimen in SEM, there is no need for ultrathin sectioning. Larger samples can be used which are often coated with metals such as gold, since biological samples are mainly insulators. The extremely thin gold coating is provided to enhance conductivity and produce a greater signal. Our BIO 222 course involves SEM specimen preparatory techniques and instruction on the proper usage of the SEM to yield a required SEM portfolio of a variety of biological and other samples.

    The main limitation to using TEM/SEM for imaging biological samples is that both microscopes operate only in a vacuum environment. This means that any biological samples observed must be dead. A biologist would certainly prefer to observe living processes at this molecular level. Since the samples must be observed under a vacuum, chemical fixation must be performed to kill and preserve the cells in as "near to life" conditions as possible.
    Although living samples cannot be studied, the ability to observe the cell through this "molecular window" has had a profound effect on the field of biology. Biological publications at all levels contain numerous electron photomicrographs in support of various ideas.

    With organizations such as the
    National Science Foundation stressing support for two-year colleges and stimulating students with innovative, "hands-on" laboratory courses, the future of electron microscopy at NCC seems bright. Current plans include writing additional equipment grants for upgrading the facility with a modern TEM and an X-ray analysis and digital computerized imaging, archiving and analysis attachment to the SEM. The immediate future plan involves the development of a SUNY registered program, similar to programs which exist at two-year colleges in California (San Joaquin Delta College) and Wisconsin (Madison Area Technical College).


    Current Major Equipment Holdings


    Electron Microscopes:

    Ultramicrotomes:

    Miscellaneous:






    Prof. Stephen Beck and Hitachi S-2400 Scanning Electron Microscope



    Prof. Beck with students Ms. Laura Richter and Mr. Mike Laux
    on the Hitachi HS-8-2 Transmission Electron Microscope


    Course Descriptions & Current Syllabi

    TRANSMISSION ELECTRON MICROSCOPY (BIO. 221)

    SCANNING ELECTRON MICROSCOPY (BIO. 222)

    Complete Course Descriptions and Current Semester Offerings Can Also Be Accessed From The NCC HomePage.

    For Additional Information, You Can Contact Prof. Stephen Beck by email or
    telephone (516) 572-7829.




    Electron Microscopy Related Links

    The following links are presented since they all have some relevance to electron and other types of microscopy. In addition to microscope manufacturers such as Hitachi, LEO, Zeiss, etc, I have included links pertaining to digital image capture and analysis hardware and software, microscope supply houses, and photographic media manufacturers (Kodak & Polaroid).


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